ELAVL1/HuR (D9W7E) Rabbit mAb

• 产品编号：CST-12582S      品牌：CST       原厂货号：12582S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

ELAVL1/HuR (D9W7E) Rabbit mAb兔单抗能够检测内源性 ELAVL1/HuR总蛋白水平。该单克隆抗体是经由合成的围绕人ELAVL1/HuR蛋白 His201位点的氨基酸肽段免疫动物而生产的。ELAVL (embryonic lethal, abnormal vision and Drosophila-like)蛋白家族包括ELAVL1/HuR, ELAVL2/HuB, ELAVL3/HuC 和 ELAVL4/HuD (1)。ELAVL1/HuR广泛表达而且其他三个成员的表达是神经元特异性的91)。 ELAVL/Hu蛋白是高度保守的RNA结合蛋白（1）。除了三个RNA识别基序外，这些蛋白还含有一个核定位信号，能够使他们在核和胞质间穿梭（2）。当用actinomycin D抑制转录时，ELAVL1/HuR会从核内重新定位于胞质内并在这里与mRNA 3' UTR上的AU-rich element结合从而稳定mRNA（3,4）。ELAVL1/HuR被认为可以通过结合mRNA来提高翻译（5,6）。另外ELAVL1/HuR 还能够与microRNA(miRNA)相互作用（7）。

1. Izquierdo, J.M. (2008) J Biol Chem 283, 19077-84.
2. Fan, X.C. and Steitz, J.A. (1998) Proc Natl Acad Sci U S A 95, 15293-8.
3. Peng, S.S. et al. (1998) EMBO J 17, 3461-70.
4. Brennan, C.M. and Steitz, J.A. (2001) Cell Mol Life Sci 58, 266-77.
5. Mazan-Mamczarz, K. et al. (2003) Proc Natl Acad Sci U S A 100, 8354-9.
6. Lal, A. et al. (2005) EMBO J 24, 1852-62.
7. Lebedeva, S. et al. (2011) Mol Cell 43, 340-52.

原厂资料：

Specificity / Sensitivity

ELAVL1/HuR (D9W7E) Rabbit mAb recognizes endogenous levels of total ELAVL1/HuR protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His201 of human ELAVL1/HuR protein.

Background

The ELAVL (embryonic lethal, abnormal vision and Drosophila-like) family of proteins includes ELAVL1/HuR, ELAVL2/HuB, ELAVL3/HuC and ELAVL4/HuD (1). ELAVL1/HuR is ubiquitously expressed whereas expression of the other three members is neuronal-specific (1). ELAVL/Hu proteins are highly conserved RNA-binding proteins (1). Besides three RNA recognition motifs, these proteins also contain nuclear localization signals that enable them to shuttle between nucleus and cytoplasm (2). Upon inhibition of transcription by actinomycin D, ELAVL1/HuR relocates from nucleus to cytoplasm where it binds the AU-rich elements within 3' UTRs to stabilize mRNAs (3, 4). ELAVL1/HuR is suggested to increase translation by binding to mRNAs (5,6). In addition, ELAVL1/HuR interacts with microRNAs (miRNAs) (7).

1. Izquierdo, J.M. (2008) J Biol Chem 283, 19077-84.
2. Fan, X.C. and Steitz, J.A. (1998) Proc Natl Acad Sci U S A 95, 15293-8.
3. Peng, S.S. et al. (1998) EMBO J 17, 3461-70.
4. Brennan, C.M. and Steitz, J.A. (2001) Cell Mol Life Sci 58, 266-77.
5. Mazan-Mamczarz, K. et al. (2003) Proc Natl Acad Sci U S A 100, 8354-9.
6. Lal, A. et al. (2005) EMBO J 24, 1852-62.
7. Lebedeva, S. et al. (2011) Mol Cell 43, 340-52.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.