Ms TH1/17 Pheotyping Kit 50Tst

• 产品编号：BD-560759      品牌：BD-Pharmingen       原厂货号：560759
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

描述：

The peripheral CD4+ T cell pool includes multiple effector and memory T cell subsets that arise through antigen-driven
expansion and differentiation of naïve T cells. The early response of naïve CD4+ T cells to antigenic stimulation is characterized
by high level proliferation and a limited cytokine repertoire. Further differentiation yields cells with a more diverse potential for
cytokine expression. Depending upon the balance of local cytokines, costimulatory molecules, antigen levels, and genetic
factors, Type-1 T helper (Th1), Th2, and Th17 effector and/or memory cells are generated by immune responses.
Functionally-polarized CD4+ T cell subsets have been identified based on their distinctive patterns of cytokine secretion. As a
signature cytokine, Th1 cells selectively produce large amounts of interferon-gamma (IFN-γ). Th2 cells selectively produce IL-4,
and Th17 express high levels of IL-17A. Through secretion of IFN-γ and other effector molecules, Th1 cells activate
macrophages, natural killer (NK) cells, and CD8+ T cells and are responsible for cell-mediated immunity. Th1 cells provide
protection against intracellular bacteria, fungi, protozoa and viruses and are involved in some autoimmune responses. IL-4
produced by Th2 cells is particularly strong in driving B cells to generate IgE-secreting cells. IgE plays a role in basophil/mast
cell mediated immune reactions. Th2 cells mediate protection against extracellular parasites but may also cause harmful allergic
responsiveness to develop. Through the secretion of IL-17A and other factors, Th17 cells recruit and activate neutrophils and
mediate immune responses against extracellular bacteria and fungi. Th17 cells are also implicated in autoimmune responses. In
addition to these types of T helper cells, Th0- (IL-4 and IFN-γ) and Th17/Th1-like (IL-17A/IFN-γ) cells that coexpress signature
cytokines have been described.
The Th1/Th17 paradigm provides a useful model system for investigating the cellular and molecular mechanisms that mediate
protective as well as harmful immune responses. The BD Mouse Th1/Th17 Phenotyping Kit provides an easy-to-use four-color
cocktail of fluorescent antibodies-specific for mouse CD4, IFN-γ (for Th1), IL-4 (for Th2) and IL-17A (for Th17)-that will
enable researchers to identify and characterize the nature of these T helper cell types by multicolor flow cytometric analysis.
The kit can be used to successfully analyze ex vivo lymphoid cell samples (eg, for the types of in vivo-generated T helper cells)
or to monitor T helper cell differentiation by cells cultured within various experimental model systems.
Investigators should note that the appearance of BD GolgiStop™ Protein Transport Inhibitor may range in color from clear
(colorless) to light yellow.

原厂资料：

注意事项：

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
The antibody was conjugated with PerCP-Cy5.5 under optimum conditions, and unconjugated antibody and free PerCP-Cy5.5 were
removed. Storage of PerCP-Cy5.5 conjugates in unoptimized diluent is not recommended and may result in loss of signal intensity.