HCA092 recognises the human molecular adaptor protein adhesion- and degranulation-promoting (ADAP). Adaptor proteins are essential components of the T-cell receptor (TCR) signalling cascade, regulating gene transcription and cytoskeletal re-organisation.
ADAP is a 130kD molecule which interacts with several intermediates of the T-cell signalling cascade including SLP-76, FYN and VASP. The ADAP molecule is expressed on haematopoietic tissues and is reported to have multiple functions downstream of the TCR that promote optimal T lymphocyte response to antigen. In particular, ADAP is reported to function as a positive regulator of T-cell activation, LFA-1 integrin clustering following TCR ligation and T-cell adhesion.
Product Form
A bivalent human recombinant Fab selected from the HuCAL® phage display library, expressed inE. coli.The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This Fab fragment is dimerized via a helix-turn-helix motif. This antibody is supplied lyophilised.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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