Gab Antibody Sampler Kit

• 产品编号：CST-9791S      品牌：Cell Signaling Technology       原厂货号：9791S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

多克隆抗体是由合成的针对人源的Gab1 蛋白Tyr472肽段或针对Gab1蛋白Tyr307或Gab2蛋白Ser159磷酸化肽段免疫动物生产的，并用蛋白质A和肽亲和层析技术纯化。单克隆抗体是由针对重组的人源的Gab2蛋白肽段或合成的人源的针对Gab1蛋白Tyr627或Gab2蛋白Tyr452磷酸化肽段免疫动物生产的。Gab Antibody Sampler Kit为您提供一个经济的手段检测Gab1和Gab2总蛋白及特定位点磷酸化的Gab1和Gab2蛋白。Kit包含足够的试剂且每个一抗可以做四次western blot 实验。Grb相关结合因子（Gab）家族是一类由众多受体酪氨酸激酶（RTKs），如EGFR，HGFR，胰岛素受体，细胞因子受体和B细胞抗原受体招募的接头蛋白。RTKs受到它们的同源配体刺激后，Gab被招募到细胞膜上，被磷酸化，并作为一个支架（1-4）。Gab1蛋白存在多个酪氨酸磷酸化位点（5）。Tyr472位点磷酸化可以调节其与PI3K激酶的p85亚基（6,7）。Gab1的Tyr307，373和407位点磷酸化可以调节Gab1与PLCγ互作（8）。Tyr627和659位点磷酸化是Gab1结合并活化蛋白酪氨酸磷酸酶SHP2所必需的（6,9）。Gab2是最近发现的Gab家族接头蛋白，包含一个pleckstrin同源结构域及潜在的含SH2和SH3结构域蛋白的结合位点。已发现gab2支持一些造血干细胞生长，分化和行使功能（10）。

1. Holgado-Madruga, M. et al. (1996) Nature 379, 560-564.
2. Weidner, K.M. et al. (1996) Nature 384, 173-176.
3. Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109-4117.
4. Ingham, R.J. et al. (2001) J Biol Chem 276, 12257-65.
5. Lehr, S. et al. (1999) Biochemistry 38, 151-159.
6. Rocchi, S. et al. (1998) Mol. Endocrinol. 12, 914-923.
7. Yu, C.F. et al. (2001) J Biol Chem 276, 32552-8.
8. Gual, P. et al. (2000) Oncogene 19, 1509-18.
9. Cunnick, J.M. et al. (2001) J Biol Chem 276, 24380-7.
10. Yi, Q. et al. (2005) Biochem Biophys Res Commun 337, 446-51.

原厂资料：

Specificity / Sensitivity

Gab1 Antibody detects endogenous levels of total Gab1 protein. Gab2 Antibody detects endogenous levels of total Gab2 protein. Each activation-state antibody detects endogenous levels of Gab1 or Gab2 phosphorylated at the specified sites. There may be some cross-reactivity with other phosphorylated proteins. Please consult individual product web pages for more details.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr472 of Gab1, or phosphopeptides corresponding to residues surrounding Tyr307 of human Gab1 or Ser159 of human Gab2. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.Monoclonal antibodies are produced by immunizing animals with recombinant human Gab2, or synthetic phosphopeptides corresponding to residues surrounding Tyr627 of human Gab1 or Tyr452 of human Gab2.

Description

The Gab Antibody Sampler Kit provides an economical means of evaluating levels of Gab1 and Gab2 total protein, as well phosphorylated Gab1 and Gab2 at the specified sites. The kit contains enough reagents to perform four western blot experiments per primary antibody.

Background

The Grb-associated binder (Gab) family is a family of adaptor proteins recruited by a wide variety of receptor tyrosine kinases (RTKs) such as EGFR, HGFR, insulin receptor, cytokine receptor and B cell antigen receptors. Upon stimulation of RTKs by their cognate ligand, Gab is recruited to the plasma membrane where it is phosphorylated and functions as a scaffold (1-4). Multiple tyrosine phosphorylation sites of Gab1 protein have been identified (5). Phosphorylation of Tyr472 regulates its binding to p85 PI3 kinase (6,7). Phosphorylation of Gab1 at Tyr307, Tyr373 and Tyr407 modulates its association to PLCγ (8). Phosphorylation of Tyr627 and Tyr659 is required for Gab1 binding to and activation of the protein tyrosine phosphatase SHP2 (6,9).Gab2, a recently identified docking protein of Gab family, contains a pleckstrin homology domain and potential binding sites for SH2 and SH3 domain-containing proteins. Gab2 has been shown to support growth, differentiation and function in a number of hematopoietic cells (10).

1. Holgado-Madruga, M. et al. (1996) Nature 379, 560-564.
2. Weidner, K.M. et al. (1996) Nature 384, 173-176.
3. Takahashi-Tezuka, M. et al. (1998) Mol. Cell. Biol. 18, 4109-4117.
4. Ingham, R.J. et al. (2001) J Biol Chem 276, 12257-65.
5. Lehr, S. et al. (1999) Biochemistry 38, 151-159.
6. Rocchi, S. et al. (1998) Mol. Endocrinol. 12, 914-923.
7. Yu, C.F. et al. (2001) J Biol Chem 276, 32552-8.
8. Gual, P. et al. (2000) Oncogene 19, 1509-18.
9. Cunnick, J.M. et al. (2001) J Biol Chem 276, 24380-7.
10. Yi, Q. et al. (2005) Biochem Biophys Res Commun 337, 446-51.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA and 50% glycerol and less than
0.02% sodium azide. Store at –20°C. Do not aliquot the
antibodies.