Pim Kinase Antibody Sampler Kit

• 产品编号：CST-9779S      品牌：Cell Signaling Technology       原厂货号：9779S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

Pim-1 (C93F2) Rabbit mAb, Pim-2 (D1D2) XP® Rabbit mAb, and Pim-3 (D17C9) Rabbit mAb 检测内源性各自的目标蛋白。Pim 抗体不与其家族中其它的成员交叉反应。 Phospho-Bad (Ser112) (40A9) Rabbit mAb 只能检测在Ser112位点磷酸化的内源 Bad 蛋白，但是不能检测在其它位点磷酸化的Bad蛋白，也不能检测其它相关家族成员。Bad (D24A9) Rabbit mAb 检测内源水平的总Bad 蛋白。此单克隆抗体是通过分别合成对应的人源 Pim-1 Val160 周围的肽段，Pim-2 Cys266周围肽段，Pim-3 Ser275周围肽段，Bad Pro102 周围肽段来免疫动物而获得。Pim Kinase Antibody Sampler Kit 为研究三个Pim激酶，Bad 和 Ser112位点磷酸化的Bad 提供了经济的方法。本试剂盒内含四次Western blots 所需的一抗和二抗。Pim 家族的蛋白 包括Pim-1, Pim-2 and Pim-3，它们是致癌基因编码的丝氨酸/苏氨酸激酶 (1). Pim-1 丝氨酸/苏氨酸激酶在造血细胞中高表达，在促有丝分裂信号传导中发挥了重要的作用并且能够快速的被多种细胞生长因子诱导 (1-4)。 Pim-1 与 c-Myc相互协作而作用在淋巴细胞转变过程中，能够保护细胞远离生长因子消退和毒素诱导的细胞凋亡(5,6)。 Pim-1 通过直接磷酸化c-Myb的DNA结合位点和转录因子共激活因子p100而增强c-Myb的转录活性 (7,8)。Pim-1基因的超活性突变体弥散在大的淋巴瘤的B-cell中(9)。 受到IL-6的诱导 Etk将Pim-1的Tyr218 位点磷酸化并增强Pim-1 的活性 (10)。 不同的Pim底物已经被鉴定，Bad 能被 Pim-1 和 Pim-2在 Ser112 位点磷酸化，这个位点的磷酸化改变了 Bad-诱导的细胞凋亡(11,12)。pim-1 基因通过不同的翻译起始位点编码两个不同的蛋白。分子量为44 kDa 的 Pim-1L和分子量为33 kDa 的 Pim-1S，它们都是活性的激酶，但是稳定性不同(13)。

1. Mikkers, H. et al. (2004) Mol Cell Biol 24, 6104-15.
2. Selten, G. et al. (1986) Cell 46, 603-11.
3. Meeker, T.C. et al. (1987) J Cell Biochem 35, 105-12.
4. Dautry, F. et al. (1988) J Biol Chem 263, 17615-20.
5. Möröy, T. et al. (1993) Proc Natl Acad Sci USA 90, 10734-8.
6. Lilly, M. and Kraft, A. (1997) Cancer Res 57, 5348-55.
7. Leverson, J.D. et al. (1998) Mol Cell 2, 417-25.
8. Winn, L.M. et al. (2003) Cell Cycle 2, 258-62.
9. Pasqualucci, L. et al. (2001) Nature 412, 341-6.
10. Kim, O. et al. (2004) Oncogene 23, 1838-44.
11. Aho, T.L. et al. (2004) FEBS Lett 571, 43-9.
12. Yan, B. et al. (2003) J Biol Chem 278, 45358-67.
13. Saris, C.J. et al. (1991) EMBO J 10, 655-64.

原厂资料：

Specificity / Sensitivity

The Pim-1 (C93F2) Rabbit mAb, Pim-2 (D1D2) XP® Rabbit mAb, and Pim-3 (D17C9) Rabbit mAb detect endogenous levels of the respective proteins. The Pim antibodies do not crossreact with other Pim family members. Phospho-Bad (Ser112) (40A9) Rabbit mAb detects endogenous levels of Bad only when phosphorylated at Ser112. It does not detect Bad phosphorylated at other sites, nor does it detect related family members. Bad (D24A9) Rabbit mAb detects endogenous levels of total Bad protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val160 of human Pim-1, Cys266 of human Pim-2, Ser275 of human Pim-3, Ser112 of mouse Bad, and Pro102 of human Bad, respectively.

Description

The Pim Kinase Antibody Sampler Kit provides an economical means to detect all three Pim kinases along with Bad and Phospho-Bad (Ser112). The kit contains enough primary and secondary antibody to perform four western blot experiments.

Background

Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases (1). Pim-1, a serine/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines (1-4). Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis (5,6). Pim-1 also enhances the transcriptional activity of c-Myb through direct phosphorylation within the c-Myb DNA binding domain as well as phosphorylation of the transcriptional coactivator p100 (7,8). Hypermutations of the Pim-1 gene are found in B-cell diffuse large cell lymphomas (9). Phosphorylation of Pim-1 at Tyr218 by Etk occurs following IL-6 stimulation and correlates with an increase in Pim-1 activity (10). Various Pim substrates have been identified; Bad is phosphorylated by both Pim-1 and Pim-2 at Ser112 and this phosphorylation reverses Bad-induced cell apoptosis (11,12).The corresponding pim-1 gene encodes a pair of proteins through use of different translation initiation sites. Both larger 44 kDa (Pim-1L) and smaller 33 kDa (Pim-1S) proteins are active kinases, but differ in stability (13).

1. Mikkers, H. et al. (2004) Mol Cell Biol 24, 6104-15.
2. Selten, G. et al. (1986) Cell 46, 603-11.
3. Meeker, T.C. et al. (1987) J Cell Biochem 35, 105-12.
4. Dautry, F. et al. (1988) J Biol Chem 263, 17615-20.
5. Möröy, T. et al. (1993) Proc Natl Acad Sci USA 90, 10734-8.
6. Lilly, M. and Kraft, A. (1997) Cancer Res 57, 5348-55.
7. Leverson, J.D. et al. (1998) Mol Cell 2, 417-25.
8. Winn, L.M. et al. (2003) Cell Cycle 2, 258-62.
9. Pasqualucci, L. et al. (2001) Nature 412, 341-6.
10. Kim, O. et al. (2004) Oncogene 23, 1838-44.
11. Aho, T.L. et al. (2004) FEBS Lett 571, 43-9.
12. Yan, B. et al. (2003) J Biol Chem 278, 45358-67.
13. Saris, C.J. et al. (1991) EMBO J 10, 655-64.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies