LAMP1 (D2D11) XP? Rabbit mAb

• 产品编号：CST-9091P      品牌：Cell Signaling Technology       原厂货号：9091P
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

LAMP1 (D2D11) XP® Rabbit mAb可以识别内源性总的LAMP1蛋白。单克隆抗体由合成的人LAMP1蛋白片段免疫动物产生。溶酶体-相关膜蛋白1和2（LAMP1和LAMP2）是两个丰度很高的溶酶体膜蛋白（1,2）。两者都是膜蛋白，位于溶酶体内腔隙在氨基端被高度糖基化，这会使蛋白免受蛋白酶解（3）。LAMP1的羧基端暴露在细胞质中，含有一个酪氨酸分选基序会靶向LAMP到溶酶体膜（4）。LAMP1和LAMP2有37%的序列同源性。LAMP1和LAMP2都涉及到调控溶酶体-吞噬体融合和胆固醇转运过程中的溶酶体活性（5,6）。

1. Eskelinen, E.L. et al. (2003) Trends Cell Biol 13, 137-45.
2. Fukuda, M. (1991) J Biol Chem 266, 21327-30.
3. Kundra, R. and Kornfeld, S. (1999) J Biol Chem 274, 31039-46.
4. Rohrer, J. et al. (1996) J Cell Biol 132, 565-76.
5. Huynh, K.K. et al. (2007) EMBO J 26, 313-24.
6. Eskelinen, E.L. et al. (2004) Mol Biol Cell 15, 3132-45.

原厂资料：

Specificity / Sensitivity

LAMP1 (D2D11) XP® Rabbit mAb recognizes endogenous levels of total LAMP1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein.

Background

Lysosome-associated membrane protein 1 and 2 (LAMP1 and LAMP2) are two abundant lysosomal membrane proteins (1,2). Both are transmembrane proteins and are heavily glycosylated at the amino-terminal luminal side of the lysosomal inner leaflet, which protects the proteins from proteolysis (3). The carboxy terminus of LAMP1 is exposed to the cytoplasm and contains a tyrosine sorting motif that targets LAMP to lysosomal membranes (4). LAMP1 and LAMP2 are 37% homologous in their protein sequences. Both LAMP1 and LAMP2 are involved in regulating lysosomal motility during lysosome-phagosome fusion and cholesterol trafficking (5,6).

1. Eskelinen, E.L. et al. (2003) Trends Cell Biol 13, 137-45.
2. Fukuda, M. (1991) J Biol Chem 266, 21327-30.
3. Kundra, R. and Kornfeld, S. (1999) J Biol Chem 274, 31039-46.
4. Rohrer, J. et al. (1996) J Cell Biol 132, 565-76.
5. Huynh, K.K. et al. (2007) EMBO J 26, 313-24.
6. Eskelinen, E.L. et al. (2004) Mol Biol Cell 15, 3132-45.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody