Rag and LAMTOR Antibody Sampler Kit

• 产品编号：CST-8665S      品牌：Cell Signaling Technology       原厂货号：8665S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

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描述：

LAMTOR1/C11orf59 (D11H6) XP ® Rabbit mAb, LAMTOR2/ROBLD3 (D7C10) Rabbit mAb, 和 LAMTOR3/MAPKSP1 (D38G5) Rabbit mAb可以识别每种内源特定蛋白的水平。另外，每一种抗体可能与其它LAMTOR蛋白发生交叉反应。 RagA (D8B5) Rabbit mAb可以识别内源性总的RagA蛋白。此抗体也可能识别内源性总的RagB蛋白，RagB抗体可以识别内源性总的RagB蛋白。RagC (D8H5) Rabbit mAb可以识别内源性总的RagC蛋白，RagD抗体可以识别内源性总的RagD蛋白。单克隆抗体是采用合成的与人类 LAMTOR1/C11orf59 蛋白的Ala71, 人类 LAMTOR2/ROBLD3 蛋白的Leu31或人类LAMTOR3/MAPKSP1蛋白的Val16位点周围的序列相对应的肽段免疫动物生产的。单克隆抗体是采用合成的与人类RagA蛋白或者RagC蛋白的氨基末端残基序列相对应的肽段免疫动物生产的。多克隆抗体是采用合成的与人类RagB和RagD 蛋白序列相对应的肽段免疫动物生产的。多克隆抗体是通过蛋白A和肽亲和层析法纯化的。Rag and LAMTOR Antibody Sampler Kit 为在mTOR复合体信号通路中检测不同的Rag和LAMTOR蛋白提供了经济的手段。本试剂盒包含一抗和二抗，可以做4次western blot。mTORC1激酶复合物是调节细胞生长的一个关键组分（1,2）。它的活性由能量水平，生长因子和氨基酸通过Akt，MAPK和AMPK通路调节（3,4）。最近的研究发现，RagA，RagB，RagC和RagD，这四个相关的GTPases与mTORC1中的raptor相互作用（1,2）。这些相互作用是mTORC1响应氨基酸信号 活化所必须的和有效的（1,2）。根据文献研究报道，RagD是在人类黑色素瘤细胞系中的几个异常表达的基因之一，已经被认为是将来诊断和治疗的重要靶点（5）。一个包括LAMTOR1/C11orf59, LAMTOR2/ROBLD3, 和 LAMTOR3/MAPKSP1的蛋白复合体，已经被认为可以与四种Rag GTP酶相互作用并且招募至溶酶体表面（6）。

1. Sancak, Y. et al. (2008) Science 320, 1496-501.
2. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
3. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
4. Wullschleger, S. et al. (2006) Cell 124, 471-84.
5. de Wit, N.J. et al. (2005) Br J Cancer 92, 2249-61.
6. Sancak, Y. et al. (2010) Cell 141, 290-303.

原厂资料：

Specificity / Sensitivity

LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb, LAMTOR2/ROBLD3 (D7C10) Rabbit mAb, and LAMTOR3/MAPKSP1 (D38G5) Rabbit mAb recognize endogenous levels of each specific protein. In addition, each antibody may cross-react with other LAMTOR proteins. RagA (D8B5) Rabbit mAb recognizes endogenous levels of total RagA protein. This antibody may also recognize endogenous levels of total RagB protein. RagB Antibody recognizes endogenous levels of total RagB protein. RagC (D8H5) Rabbit mAb recognizes endogenous levels of total RagC protein. RagD Antibody recognizes endogenous levels of total RagD protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala71 of human LAMTOR1/C11orf59 protein, residues surrounding Leu31 of human LAMTOR2/ROBLD3 protein, or residues surrounding Val16 of human LAMTOR3/MAPKSP1 protein. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human RagA protein or residues in the amino terminus of human RagC protein. Polyclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human RagB or RagD proteins. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Rag and LAMTOR Antibody Sampler Kit is an economical means of detecting various Rag and LAMTOR proteins implicated within mTOR complex signaling. The kit contains enough primary and secondary antibody to perform four western blots with each antibody.

Background

The mTORC kinase complex is a critical regulator of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids via signaling through Akt, MAPK, and AMPK pathways (3,4). Recent studies found that the four related GTPases, RagA, RagB, RagC, and RagD, interact with raptor within the mTORC1 complex (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). According to the research literature, RagD is one of several genes differentially expressed in human melanoma cell lines and has been considered to be a viable target for further diagnostic and therapeutic study (5). A protein complex consisting of LAMTOR1/C11orf59, LAMTOR2/ROBLD3, and LAMTOR3/MAPKSP1 has been identified to interact with and recruit four Rag GTPases to the surface of lysosomes (6).

1. Sancak, Y. et al. (2008) Science 320, 1496-501.
2. Kim, E. et al. (2008) Nat Cell Biol 10, 935-45.
3. Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45.
4. Wullschleger, S. et al. (2006) Cell 124, 471-84.
5. de Wit, N.J. et al. (2005) Br J Cancer 92, 2249-61.
6. Sancak, Y. et al. (2010) Cell 141, 290-303.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.