Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb

• 产品编号：CST-8438P      品牌：Cell Signaling Technology       原厂货号：8438P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb 兔单抗仅能够检测内源性的Asp198磷酸化的Caspase-7蛋白。该单克隆抗体是采用合成的人源caspase-7蛋白Asp198 位周围残基相对应的肽段免疫动物而制备的。Caspase-7（CMH-1、Mch3、ICE-LAP3），已被确定为是细胞凋亡的主要执行者（1-4）。 Caspase-7，和caspase-3一样，是半胱天冬酶效应子，负责裂解下游底物，如（ADP-核糖）聚合酶和PARP（1,3）。在细胞凋亡中，caspase-7被上游 caspases的蛋白水解过程激活ASP23，Asp198和Asp206位点，从而产生成熟的亚基（1,3）。类似caspase -2和-3，caspase-7优先切割识别序列DEVD后的底物（5）。

1. Fernandes-Alnemri, T. et al. (1995) Cancer Res 55, 6045-52.
2. Duan, H. et al. (1996) J Biol Chem 271, 1621-5.
3. Lippke, J.A. et al. (1996) J Biol Chem 271, 1825-8.
4. Cohen, G.M. (1997) Biochem J 326 ( Pt 1), 1-16.
5. Thornberry, N.A. et al. (1997) J Biol Chem 272, 17907-11.

原厂资料：

Specificity / Sensitivity

Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb recognizes endogenous levels of caspase-7 protein only when cleaved at Asp198.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp198 of human caspase-7 protein.

Background

Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP (1,3). During apoptosis, caspase-7 is activated through proteolytic processsing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits (1,3). Similar to caspases-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD (5).

1. Fernandes-Alnemri, T. et al. (1995) Cancer Res 55, 6045-52.
2. Duan, H. et al. (1996) J Biol Chem 271, 1621-5.
3. Lippke, J.A. et al. (1996) J Biol Chem 271, 1825-8.
4. Cohen, G.M. (1997) Biochem J 326 ( Pt 1), 1-16.
5. Thornberry, N.A. et al. (1997) J Biol Chem 272, 17907-11.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.