Phospho-DR6 (Ser562) Antibody

• 产品编号：CST-7616S      品牌：CST       原厂货号：7616S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-DR6 (Ser562) Antibody 只能够检测内源水平的在Ser562磷酸化的DR6蛋白。该抗体或许能够识别Ser562和Ser565双位点磷酸化的DR6蛋白。该多克隆抗体是采用合成的人源DR6蛋白Ser562周围残基相对应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。肿瘤坏死因子受体家族，包括TNF-RI，FAS，DR3，DR4，DR5和DR6，在各个生理系统细胞凋亡的调控中起着重要的作用（1,2）。受体被一个细胞因子家族激活，包括TNF、FasL和TRAIL。它们的特点是含有一个高度保守、富含半胱氨酸的重复序列的胞外区，和一个胞内保守的约80个氨基酸的死亡结构域（DD）。 DD对于死亡信号转导的传递很重要，它通过招募其他的DD配体蛋白（FADD，TRADD，RIP）到死亡诱导信号复合物（DISC）来发挥作用，进而激活半胱天冬酶蛋白。DR6，也被称为TNFRSF21，是肿瘤坏死因子受体家族成员，能够诱导细胞凋亡及激活NF-κB和JNK（3）。 DR6的表达被NF-κB信号上调（4）。DR6在活化和分化T细胞和B淋巴细胞中发挥了关键作用（5,6）。在神经系统中，β-淀粉样蛋白前体蛋白（APP）激活DR6从而触发神经元变性（7）。Phospho-DR6 (Ser562) Antibody直接针对一个被CST公司通过PhosphoScan®——专有修饰位点发现LC-MS/MS平台并使用一个Akt substrate antibody鉴定的位点（8）。更多信息，请访问PhosphoSitePlus®，我们修饰位点知识库，www.phosphosite.org。

1. Nagata, S. (1997) Cell 88, 355-365.
2. Thorburn, A. (2004) Cell. Signal. 16, 139-144.
3. Pan, G. et al. (1998) FEBS Lett 431, 351-6.
4. Kasof, G.M. et al. (2001) Oncogene 20, 7965-75.
5. Zhao, H. et al. (2001) J Exp Med 194, 1441-8.
6. Schmidt, C.S. et al. (2003) J Exp Med 197, 51-62.
7. Nikolaev, A. et al. (2009) Nature 457, 981-9.
8. Moritz, A. et al. (2010) Sci Signal 3, ra64.

原厂资料：

Specificity / Sensitivity

Phospho-DR6 (Ser562) Antibody recognizes endogenous levels of DR6 protein only when phosphorylated at Ser562. This antibody may recognize DR6 with dual phosphorylation at Ser562 and Ser565.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser562 of human DR6 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, and TRAIL. They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC), resulting in activation of caspases.DR6, also known as TNFRSF21, is a TNFR family member able to induce apoptosis as well as activation of NF-κB and JNK (3). Expression of DR6 is upregulated by NF-κB signaling (4). DR6 appears to play a critical role in the activation and differentiation of T and B lymphocytes (5,6). In the nervous system β-amyloid precursor protein (APP) activates DR6 to trigger neuronal degeneration (7). 
 Phospho-DR6 (Ser562) Antibody is directed at a site that was identified at Cell Signaling Technology (CST) using PhosphoScan®, our proprietary LC-MS/MS platform for modification site discovery using an Akt substrate antibody (8). Please visit PhosphoSitePlus®, our modification site knowledgebase, at www.phosphosite.org for more information.

1. Nagata, S. (1997) Cell 88, 355-365.
2. Thorburn, A. (2004) Cell. Signal. 16, 139-144.
3. Pan, G. et al. (1998) FEBS Lett 431, 351-6.
4. Kasof, G.M. et al. (2001) Oncogene 20, 7965-75.
5. Zhao, H. et al. (2001) J Exp Med 194, 1441-8.
6. Schmidt, C.S. et al. (2003) J Exp Med 197, 51-62.
7. Nikolaev, A. et al. (2009) Nature 457, 981-9.
8. Moritz, A. et al. (2010) Sci Signal 3, ra64.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.