HIPK2 Antibody

• 产品编号：CST-5091S      品牌：CST       原厂货号：5091S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HIPK2 Antibody 能够检测内源性的HIPK2总蛋白。该多克隆抗体是采用合成的人源HIPK2蛋白Gln1045周围残基相对应的肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。同源域互作蛋白激酶家族成员（HIPK1-4）的丝氨酸/苏氨酸激酶，能够在细胞增殖，分化和凋亡过程中调控基因的转录（1-3）。 HIPK1-3是核蛋白质，最初把它描述为同源盒转录因子的共抑制子（1）。HIPK蛋白和/或转录调节子相互发生磷酸化作用（4）。HIPK2在应答包括紫外线辐射和化疗药物在内的DNA损伤时被激活，磷酸化p53的Ser46，促使促凋亡p53靶基因的转录（5-7）。此外，HIPK2与一些转录因子相互作用，调控发育过程、肿瘤抑制和细胞凋亡（4）。该激酶受SUMO化（8）和泛素化（9,10）的调控。泛素化引起随后HIPK2的降解，这能够被DNA损伤剂抑制。Caspase依赖的HIPK2裂解，切除了抑制域，导致HIPK2活性增强（11）。

1. Kim, Y.H. et al. (1998) J Biol Chem 273, 25875-9.
2. Rochat-Steiner, V. et al. (2000) J Exp Med 192, 1165-74.
3. Arai, S. et al. (2007) FEBS Lett 581, 5649-57.
4. Rinaldo, C. et al. (2007) Biochem Cell Biol 85, 411-8.
5. Hofmann, T.G. et al. (2002) Nat Cell Biol 4, 1-10.
6. D'Orazi, G. et al. (2002) Nat Cell Biol 4, 11-9.
7. Di Stefano, V. et al. (2004) Exp Cell Res 293, 311-20.
8. Kim, Y.H. et al. (1999) Proc Natl Acad Sci U S A 96, 12350-5.
9. Choi, D.W. et al. (2008) J Biol Chem 283, 4682-9.
10. Winter, M. et al. (2008) Nat Cell Biol 10, 812-24.
11. Gresko, E. et al. (2006) EMBO J 25, 1883-94.

原厂资料：

Specificity / Sensitivity

HIPK2 Antibody detects endogenous levels of total HIPK2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln1045 of human HIPK2 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Background

Members of the homeodomain-interacting protein kinase (HIPK1-4) family of serine/threonine kinases regulate gene transcription with effects on cell proliferation, differentiation, and apoptosis (1-3). HIPK1-3 are nuclear proteins that were originally described as co-repressors for homeobox transcription factors (1). HIPK proteins can interact with and/or phosphorylate many transcriptional regulators (4).HIPK2 activated in response to DNA damage, including UV radiation and chemotherapeutic drugs, phosphorylates p53 at Ser46 to promote the transcription of pro-apoptotic p53 target genes (5-7). In addition, HIPK2 interacts with a number of transcription factors that control developmental processes, tumor suppression and apoptosis (4). The kinase is regulated by both sumoylation (8) and ubiquitination (9,10). Ubiquitination and subsequent degradation of HIPK2 is inhibited by DNA damaging agents. Caspase-dependent cleavage of HIPK2 removes the inhibitory domain and results in enhanced HIPK2 activity (11).

1. Kim, Y.H. et al. (1998) J Biol Chem 273, 25875-9.
2. Rochat-Steiner, V. et al. (2000) J Exp Med 192, 1165-74.
3. Arai, S. et al. (2007) FEBS Lett 581, 5649-57.
4. Rinaldo, C. et al. (2007) Biochem Cell Biol 85, 411-8.
5. Hofmann, T.G. et al. (2002) Nat Cell Biol 4, 1-10.
6. D'Orazi, G. et al. (2002) Nat Cell Biol 4, 11-9.
7. Di Stefano, V. et al. (2004) Exp Cell Res 293, 311-20.
8. Kim, Y.H. et al. (1999) Proc Natl Acad Sci U S A 96, 12350-5.
9. Choi, D.W. et al. (2008) J Biol Chem 283, 4682-9.
10. Winter, M. et al. (2008) Nat Cell Biol 10, 812-24.
11. Gresko, E. et al. (2006) EMBO J 25, 1883-94.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.