Phospho-YAP (Ser127) Antibody

• 产品编号：CST-4911S      品牌：CST       原厂货号：4911S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

磷酸化YAP（Ser127）抗体只识别内源性的Ser127磷酸化的YAP蛋白。多克隆抗体由合成磷酸化肽段免疫动物，该合成肽段与YAP人源蛋白邻近Ser127的氨基酸残基的序列一致，抗体由蛋白A和肽段亲和层析技术纯化。YAP蛋白(Yes相关蛋白，YAP65)因其与Yes的SH3结构域结合而被鉴定，它也会结合含有SH3结构域的其他蛋白，如Nck, Crk, Src, 和Abl（1）。除了SH3结合基序，YAP 还含有一个PDZ相互作用基序、卷曲螺旋结构域和WW结构域（2-4）。YAP的早期研究都指出它在锚定和靶定特异细胞组合时起作用，随后还发现YAP是转录共激活因子，其机理主要是是通过YAP的WW结构域与PEBP2转录因子和其他转录因子的PY基序(PPxY)相互作用（5,6）。由于在丝氨酸127的磷酸化，YAP蛋白依赖于AKT的与14-3-3蛋白相互作用，进而抑制p73介导的细胞凋亡（6）。

1. Sudol, M. (1994) Oncogene 9, 2145-52.
2. Mohler, P.J. et al. (1999) J Cell Biol 147, 879-90.
3. Espanel, X. and Sudol, M. (2001) J Biol Chem 276, 14514-23.
4. Sudol, M. et al. (1995) FEBS Lett 369, 67-71.
5. Yagi, R. et al. (1999) EMBO J 18, 2551-62.
6. Basu, S. et al. (2003) Mol Cell 11, 11-23.
7. Zhao, B. et al. (2010) Genes Dev 24, 862-74.
8. Zhao, B. et al. (2007) Genes Dev 21, 2747-61.
9. Yu, F.X. et al. (2012) Cell 150, 780-91.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey, Bovine

Specificity / Sensitivity

Phospho-YAP (Ser127) Antibody detects endogenous levels of YAP only when phosphorylated at serine 127.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser127 of human YAP. Antibodies are purified by protein A and peptide affinity chromatography.

Background

YAP (Yes-associated protein, YAP65) was identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl (1). In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains (2-4). While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors (5,6). Upon phosphorylation at Ser127, YAP interacts with 14-3-3 in an Akt-dependent manner and suppresses p73-mediated apoptosis (6).

1. Sudol, M. (1994) Oncogene 9, 2145-52.
2. Mohler, P.J. et al. (1999) J Cell Biol 147, 879-90.
3. Espanel, X. and Sudol, M. (2001) J Biol Chem 276, 14514-23.
4. Sudol, M. et al. (1995) FEBS Lett 369, 67-71.
5. Yagi, R. et al. (1999) EMBO J 18, 2551-62.
6. Basu, S. et al. (2003) Mol Cell 11, 11-23.
7. Zhao, B. et al. (2010) Genes Dev 24, 862-74.
8. Zhao, B. et al. (2007) Genes Dev 21, 2747-61.
9. Yu, F.X. et al. (2012) Cell 150, 780-91.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.