Phospho-Glycogen Synthase (Ser641) Antibody

• 产品编号：CST-3891S      品牌：CST       原厂货号：3891S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Glycogen Synthase (Ser641) Antibody可同时检测内源性肌肉和肝脏的在640位或641位磷酸化的糖元合成酶。多克隆抗体是通过合成对应于人肝脏GS蛋白Ser641序列的肽段免疫动物获得，抗体经Protein A和肽亲和层析纯化制得。糖元合成酶（GS）是糖元合成调节中的关键酶。哺乳动物有两种GS同工酶：肌肉GS和肝脏GS（1－2）。肌肉GS在多个组织中都有表达，而肝脏GS似乎是组织特异性的（3）。GS受到多个位点磷酸化的调控，同时也受变构配体结合的调控。磷酸化会抑制GS活性，但活性可被变异配体6-磷酸葡萄糖结合后恢复，6-磷酸葡萄糖可以诱导GS的去磷酸化从而激活。Ser641是糖元合成酶最重要的磷酸化调控位点。

1. Browner, M.F. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 1443-1447.
2. Bai, G. et al. (1990) J. Biol. Chem. 265, 7843-7848.
3. Roach, P.J. et al. (1998) J. Basic Clin. Physiol. Pharmacol. 9, 139-151.
4. Villar-Palasi, C. and Guinovart, J.J. (1997) FASEB J. 11, 544-558.
5. Skurat, A.V. and Roach, P.J. (1995) J. Biol. Chem. 270, 12491-12497.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Horse

Specificity / Sensitivity

Phospho-Glycogen Synthase (Ser641) Antibody detects endogenous levels of both muscle and liver isoforms of glycogen synthase only when phosphorylated at serine 640 or 641 of the muscle and liver isoforms, respectively.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser641 of human liver glycogen synthase. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Glycogen synthase (GS) is a key enzyme in the regulation of glycogen synthesis. There are two main isoforms of mammalian GS: the muscle and the liver isoform (1-2). Muscle GS is expressed in several tissues, and liver GS appears to be tissue specific (3). GS is regulated by multi-site phosphorylation and by the binding of allosteric ligands. Phosphorylation leads to inactivation of GS, but activity is restored by the binding of the allosteric activator glucose-6-phosphate, which induces its dephosphorylation and activation (4).Ser641 is one of the most important phosphorylation sites for the regulation of glycogen synthase (5).

1. Browner, M.F. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 1443-1447.
2. Bai, G. et al. (1990) J. Biol. Chem. 265, 7843-7848.
3. Roach, P.J. et al. (1998) J. Basic Clin. Physiol. Pharmacol. 9, 139-151.
4. Villar-Palasi, C. and Guinovart, J.J. (1997) FASEB J. 11, 544-558.
5. Skurat, A.V. and Roach, P.J. (1995) J. Biol. Chem. 270, 12491-12497.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5),
150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at
–20°C. Do not aliquot the antibody.