CD10 (CB-CALLA) Mouse mAb

• 产品编号：CST-3565S      品牌：CST       原厂货号：3565S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CD10 (CB-CALLA) Mouse mAb 能够检测内源水平 CD10蛋白。此单克隆抗体是通过CALLA细胞免疫BALB/c小鼠而获得。CD10 (CALLA或脑啡肽酶)被鉴定在在儿童急性淋巴细胞白血病中作为标志的白血病细胞，它属于胞外酶家族，包括氨基肽酶A，N和二基肽酶Ⅳ(1-2)。CD10是一个跨膜II型分子并作为金属肽酶需要锌而发挥作用。尤其, CD10剪切1-3氨基末端氨基酸，更为倾向于剪切中性的氨基酸(缬氨酸，异亮氨酸，苯丙氨酸，亮氨酸和丙氨酸) (2)。CD10 抗原的表达是通过未成熟的生发中心B细胞，由粒细胞和急性B淋巴细胞白血病细胞表达。因此，CD10 可能是参与了后期对造血干细胞的调控(3)。

1. Greaves , M. F. et al. (1975) Clin. Immunol. Immunopathol. 4, 67-84.
2. Shipp, M.A. and Look, A.T. (1993) Blood 82, 1052-1070.
3. Bene, M. C. et al. (1997) Haematologica. 82, 205-210.

原厂资料：

Specificity / Sensitivity

CD10 (CB-CALLA) Mouse mAb detects endogenous levels of CD10.

Source / Purification

Monoclonal antibody is produced by immunizing BALB/c mice with CALLA cells.

Background

CD10 (CALLA or neprilysin) was identified as a marker for leukemic cells in children with acute lymphoblastic leukemia and belongs to a family of exoenzymes that include aminopeptidase A, N and dipeptidylpeptidase IV (1-2). CD10 is a transmembrane type II molecule and functions as a metallo-peptidase requiring zinc. Specifically, CD10 cleaves 1-3 amino-terminal amino acids from peptides with a preference for neutral amino acids (valine, iso-leucine, phenylalanine, leucine or alanine) (2). The CD10 antigen is expressed by immature and by germinal center B cells, by granulocytes and by acute B-cell lymphoblastic leukemia cells. Therefore, CD10 appears to be involved in late stage regulation of hematopoietic cells (3).

1. Greaves , M. F. et al. (1975) Clin. Immunol. Immunopathol. 4, 67-84.
2. Shipp, M.A. and Look, A.T. (1993) Blood 82, 1052-1070.
3. Bene, M. C. et al. (1997) Haematologica. 82, 205-210.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.