Atg3 Antibody

• 产品编号：CST-3415S      品牌：CST       原厂货号：3415S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Atg3 Antibody 能够检测内源性的Atg3总蛋白。该多克隆抗体通过合成肽免疫动物制备，合成肽的序列与Atg3氨基末端周围的残基相一致。抗体通过蛋白A和多肽亲和色谱法纯化。自噬是自噬溶酶体对其包裹的细胞质内容物进行降解的一种分解代谢过程(1)。关于自噬调控的研究，大部分集中在酵母中，还包含一些由自噬相关基因(Atg)编码的蛋白。自噬小泡的形成，需要一对重要的泛素样连接体系，Atg12-Atg5和Atg8-磷脂酰乙醇胺（Atg8-PE），这些广泛存在于真核细胞中(2)。Atg8与其在哺乳动物中的同源染色体的结合需要Atg3的参与(酵母中的Apg3p/Aut1p)，Atg3是一种普遍表达E2样连接酶(5-7)。C端被半胱氨酸蛋白酶Atg4裂解后，Atg8暴露的甘氨酸残基与E1样连接酶Atg7结合，并转移到Atg3处，接着与磷酸酯乙醇胺结合。Atg3缺陷的小鼠会在出生后1天内死亡，并且完全缺乏结合Atg8同源物以及自噬体形成的能力(8)。

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
2. Ohsumi, Y. (2001) Nat Rev Mol Cell Biol 2, 211-6.
3. Kabeya, Y. et al. (2000) EMBO J 19, 5720-8.
4. Kabeya, Y. et al. (2004) J Cell Sci 117, 2805-12.
5. Tanida, I. et al. (2002) J Biol Chem 277, 13739-44.
6. Ichimura, Y. et al. (2000) Nature 408, 488-92.
7. Schlumpberger, M. et al. (1997) J Bacteriol 179, 1068-76.
8. Sou, Y.S. et al. (2008) Mol Biol Cell 19, 4762-75.

原厂资料：

Specificity / Sensitivity

Atg3 Antibody detects endogenous levels of total Atg3 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of Atg3. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (1). The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related genes (Atg). Formation of the autophagic vesicles involves two ubiquitin-like conjugation systems, Atg12-Atg5 and Atg8-phosphatidylethanolamine (Atg8-PE), which are essential for autophagy and widely conserved in eukaryotes (2). There are at least three Atg8 homologs in mammalian cells, GATE-16, GABARAP, and LC3, that are conjugated by lipids (3,4). Lipid conjugation of Atg8 and its mammalian homologs requires Atg3 (Apg3p/Aut1p in yeast), an ubiquitously expressed E2-like enzyme (5-7). Following C-terminal cleavage by the cysteine protease Atg4, the exposed glycine residue of Atg8 binds to the E1-like enzyme Atg7, is transferred to Atg3, and then conjugated to phophatidylethanolamine. Atg3-deficient mice die within 1 day after birth and are completely defective for the conjugation of Atg8 homlogs and autophagome formation (8).

1. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
2. Ohsumi, Y. (2001) Nat Rev Mol Cell Biol 2, 211-6.
3. Kabeya, Y. et al. (2000) EMBO J 19, 5720-8.
4. Kabeya, Y. et al. (2004) J Cell Sci 117, 2805-12.
5. Tanida, I. et al. (2002) J Biol Chem 277, 13739-44.
6. Ichimura, Y. et al. (2000) Nature 408, 488-92.
7. Schlumpberger, M. et al. (1997) J Bacteriol 179, 1068-76.
8. Sou, Y.S. et al. (2008) Mol Biol Cell 19, 4762-75.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.