p18 INK4C (DCS118) Mouse mAb

• 产品编号：CST-2896P      品牌：Cell Signaling Technology       原厂货号：2896P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

The p18 INK4C (DCS118) Mouse mAb能够检测内源性p18 INK4C 蛋白，该抗体不与其他INK4家族蛋白发生交叉反应。该单克隆抗体是由纯化重组的人p18 INK4C蛋白免疫动物生产的。周期蛋白依赖性激酶(CDKs)的激活部分经由和周期蛋白形成复合体。例如，CDK4和CDK6联合D-型周期蛋白并磷酸化Rb蛋白。该磷酸化过程是细胞进入S期必不可少的事件(1)。CDK4(INK4)抑制剂家族包括p15 INK4B, p16 INK4A, p18 INK4C和p19 INK4D。p18被认为是一个在体的单纯不足的肿瘤抑制基因(2)。所有的INK4蛋白都是由32个氨基酸残基铆钉蛋白模体组成并能够选择性的抑制CDK4/6的活性。p18的突变分析表明第四个锚定蛋白的第三个氨基末端部分介导CDK4/6的结合(3)。INK4家族成员的相互作用可能是通过与CDK4/6形成的二元复合体或者是周期蛋白D结合CDK4/6形成的三元复合体并最终导致细胞周期进程的抑制(4,5)。

1. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925.
2. Bai, F. et al. (2003) Mol. Cell. Biol. 23, 1269-1277.
3. Noh, S.J. et al. (1999) Cancer Res. 59, 558-564.
4. Guan, K.L. et al. (1994) Genes Dev. 8, 2939-2952.
5. Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.

原厂资料：

Specificity / Sensitivity

The p18 INK4C (DCS118) Mouse mAb detects endogenous levels of p18 INK4C protein. The antibody does not cross-react with other proteins of the INK4 family.

Source / Purification

Monoclonal antibody is produced by immunizing animals with purified recombinant human p18 INK4C protein.

Background

Cyclin-dependent kinases (CDKs) are activated in part by forming complexes with cyclins. For example, CDK4 and CDK6 associate with the D-type cyclins and phosphorylate the retinoblastoma protein. This phosphorylation is a necessary event for cells to enter S-phase (1). The inhibitors of CDK4 (INK4) family include p15 INK4B, p16 INK4A, p18 INK4C and p19 INK4D. p18 has been shown to function as a haploinsufficient tumor suppressor in vivo (2). All INK4 proteins are composed of 32 amino acid ankyrin motifs and selectively inhibit CDK4/6 activity. Mutational analyses of p18 implicate the third and the amino-terminal portion of the fourth ankyrin repeat in mediating binding to CDK4/6 (3). The interaction of INK4 family members can be a binary complex with CDK4/6 or ternary complex with cyclin D-bound CDK4/6 and ultimately results in the inhibition of cell cycle progression (4,5).

1. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-6925.
2. Bai, F. et al. (2003) Mol. Cell. Biol. 23, 1269-1277.
3. Noh, S.J. et al. (1999) Cancer Res. 59, 558-564.
4. Guan, K.L. et al. (1994) Genes Dev. 8, 2939-2952.
5. Hirai, H. et al. (1995) Mol. Cell. Biol. 15, 2672-2681.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.