eEF1A Antibody

• 产品编号：CST-2551S      品牌：CST       原厂货号：2551S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

eEF1A Antibody检测内源性eEF1A总蛋白水平。通过人工合成人源eEF1A蛋白羧基末端相应序列的多肽片段，使用该片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。翻译是氨基酸在核糖体上装配成多肽的过程。这个过程通常分为翻译的起始、延伸和终止三个阶段。在延伸过程中，mRNA和tRNA在核糖体的两个活化位点(A and P sites)配对。在哺乳动物细胞系中，许多真核延伸因子（eukaryotic elongation factors，eEFs)涉及到这个延伸过程(1)。eEF1A又称延伸因子Tu (EF-Tu)结合GTP并且和氨基酰- tRNAs相互作用，从而促进氨基酰- tRNAs招募到核糖体的A位点(1)。在GTP水解之后，GDP-eEF1A离开核糖体，并且通过eEF1B将其转变成GTP-eEF1A (1)。研究证明eEF1A蛋白在一定的条件下被磷酸化，显示该蛋白的活性是在翻译后水平被调控的(2,3)。

1. Browne, G.J. and Proud, C.G. (2002) Eur J Biochem 269, 5360-8.
2. Venema, R.C. et al. (1991) J Biol Chem 266, 12574-80.
3. Venema, R.C. et al. (1991) J Biol Chem 266, 11993-8.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Xenopus

Specificity / Sensitivity

eEF1A Antibody detects endogenous levels of total eEF1A protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence at the C-terminal end of human eEF1A. Antibodies are purified by peptide affinity chromatography.

Background

Translation is the process where amino acid residues are assembled into polypeptides on ribosomes. This process is generally divided into three stages: initiation, elongation and termination. During elongation, mRNA and tRNA pair at the two active sites (A and P sites) on the ribosome. A number of eukaryotic elongation factors (eEFs) are involved in this process in mammalian cells (1). eEF1A, also called elongation factor Tu (EF-Tu), binds GTP and interacts with amino acyl-tRNAs to promote recruitment of amino acyl-tRNAs to the A-site of the ribosome (1). After GTP hydrolysis, GDP-eEF1A leaves the ribosome and is later converted back to the GTP-eEF1A by eEF1B (1). Studies have shown that eEF1A is phosphorylated under certain conditions, indicating that its activity is regulated at the post-translational level (2,3).

1. Browne, G.J. and Proud, C.G. (2002) Eur J Biochem 269, 5360-8.
2. Venema, R.C. et al. (1991) J Biol Chem 266, 12574-80.
3. Venema, R.C. et al. (1991) J Biol Chem 266, 11993-8.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.