Cytochrome c (D18C7) Rabbit mAb?(HRP Conjugate)

• 产品编号：CST-12959S      品牌：CST       原厂货号：12959S
• 产品分类：抗体 > 一抗 > 酶标记一抗 > 辣根过氧化物酶（HRP）
• 应用分类：

描述：

Cytochrome c (D18C7) Rabbit mAb (HRP Conjugate)兔单抗可以识别内源水平的细胞色素C总蛋白。该单克隆抗体是采用合成的人细胞色素C蛋白Pro72周围残基相对应的肽段免疫动物生产的。该CST抗体与辣根过氧化物酶（HRP）的碳水化合物基团偶联。HRP偶联的抗体被认为与未偶联的Cytochrome c (D18C7) Rabbit mAb 兔单抗#11940具有相同的交叉反应性。细胞色素C是一种保守的电子传递蛋白，作为呼吸链的组分存在于于线粒体内膜中(1)。受到凋亡刺激信号后，细胞色素C从线粒体释放， 并与procaspase-9 (47 kDa)/Apaf 1交联。此交联复合物促使caspase-9从没有活性的酶原转变成活化状态(2)。这一过程将进一步激发caspase-3的活化最终导致凋亡(3)。

1. Schagger H.H. et al. (2002) Biochem. Biophys. Acta. 1555, 154-159.
2. Li, P. et al. (1997) Cell 91, 479-489.
3. Liu, X. et al. (1996) Cell 86, 147-157.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine, Pig

Specificity / Sensitivity

Cytochrome c (D18C7) Rabbit mAb (HRP Conjugate) recognizes endogenous levels of total cytochrome c protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro72 of human cytochrome c protein.

Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cytochrome c (D18C7) Rabbit mAb #11940.

Background

Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3).

1. Schagger H.H. et al. (2002) Biochem. Biophys. Acta. 1555, 154-159.
2. Li, P. et al. (1997) Cell 91, 479-489.
3. Liu, X. et al. (1996) Cell 86, 147-157.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50%
glycerol. Store at –20°C. Do not aliquot the antibodies.