C/EBP Antibody Sampler Kit

• 产品编号：CST-12814S      品牌：Cell Signaling Technology       原厂货号：12814S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

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描述：

除非有特别指明，每个抗体都能检测其内源性的靶蛋白。每个激活状态的抗体都可以识别它磷酸化的靶蛋白。Phospho-C/EBPβ (Thr235) Antibody可以识别Thr235磷酸化的内源性human liver activating protein (LAP)，Thr188被磷酸化的小鼠和大鼠LAP，以及Thr37被磷酸化的liver inhibitory protein (LIP)。C/EBPβ (LAP)能够检测到内源性的C/EBPβ, the p38 和 p36 LAPs, 但是不会检测到p20 LIP。由合成磷酸肽段免疫动物获得多克隆抗体，磷酸肽段分别与human C/EBPα的Ser21，mouse C/EBPα的Thr222/226或human C/EBPβ的Thr235邻近氨基酸序列一致。由合成肽段免疫动物获得多克隆抗体，肽段分别与human C/EBPβ或mouse C/EBPδ的氨基端序列一致。多抗经过蛋白A和肽段亲和层析纯化。使用合成的肽段免疫动物获得单抗，单抗分别和human C/EBPα的Ala176或human CHOP的Leu159邻近氨基酸残基序列一致。C/EBP Antibody Sampler 试剂盒提供了经济的方式检测转录因子C/EBP家族和一些参与它的活化的磷酸化位点。试剂盒提供了足以进行4次western blot实验的一抗。CCAAT/增强子结合蛋白(C/EBP)是对细胞分化，终端功能和炎症反应有关键的转录因子。六个特征家族成员C/EBPα, β, δ, γ, ε, and ζ)分布在各种组织中(1)。从不同的起始密码子开始翻译产生的两个C/EBPα亚型(p42和p30)是强转录激活子(2)。研究指出胰岛素和胰岛素样生长因子-I刺激C/EBPα去磷酸化，其可在胰岛素诱导的GLUT4转录抑制过程中发挥作用(3)。GSK-3磷酸化C/EBPα的Thr222, Thr226,和Ser230可能为脂肪形成过程所需(4)。C/EBPβ的两个形式，38 kDa的liver activating protein (LAP) 和 20 kDa的 liver inhibitory protein (LIP)，可能来自于选择性翻译。38kDa的LAP蛋白是一个转录激活蛋白而LIP做可能会抑制C/EBPβ的转录活性(5). C/EBPβ的不同位点被磷酸化会激活其转录活性(6-8)。C/EBPβ的大鼠特异性Ser105被磷酸化在大鼠中对于C/EBPβ的活化是必须的(9)。C/EBPδ在脂肪组织，肺和小肠中高表达(10)。脂肪形成过程中C/EBPδ的mRNA水平表达增高提示C/EBPδ能够正向调控脂肪形成(10,11)。C/EBPδ在哺乳动物神经系统中表达并在长期记忆中发挥了重要作用(10,12)。CHOP是一个C/EBP-同源蛋白能够以显性抑制的方式抑制C/EBP和LAP(13)。CHOP的表达是被细胞压力所诱导表达的，包括饥饿；表达的CHOP抑制细胞周期进展阻止G1进入S期(14)。ER压力期，CHOP表达升高并发挥介导细胞程序性死亡的功能(15)。

原厂资料：

Specificity / Sensitivity

Unless otherwise indicated, each antibody will recognize endogenous total levels of their target protein. Each activation state antibody recognizes the phosphorylated form of its target. Phospho-C/EBPβ (Thr235) Antibody recognizes endogenous levels of human liver activating protein (LAP) only when phosphorylated at Thr235, mouse and rat LAP only when phosphorylated at Thr188, and liver inhibitory protein (LIP) only when phosphorylated at Thr37. The C/EBPβ (LAP) Antibody detects endogenous levels of total C/EBPβ, the p38 and p36 LAPs, but not the p20 LIP.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser21 of human C/EBPα, Thr222/226 of mouse C/EBPα, or Thr235 of human C/EBPβ. Polyclonal antibodies are also produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human C/EBPβ or the sequence of mouse C/EBPδ. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala176 of human C/EBPα protein or to residues surrounding Leu159 of human CHOP protein.

Description

The C/EBP Antibody Sampler Kit provides an economical means of evaluating the C/EBP family of transcription factors and several phosphorylation sites that are involved in its activation. The kit includes enough antibody to perform four western blot experiments with each primary antibody.

Background

CCAAT/enhancer-binding proteins (C/EBPs) are transcription factors critical for cellular differentiation, terminal function, and inflammatory response. Six characterized family members (C/EBPα, β, δ, γ, ε, and ζ) are distributed in a variety of tissues (1). Translation from alternative start codons results in two C/EBPα isoforms (p42 and p30) that are strong transcriptional activators (2). Research studies indicate that insulin and insulin-like growth factor-I stimulate C/EBPα dephosphorylation, which may play a key role in insulin-induced repression of GLUT4transcription (3). Phosphorylation of C/EBPα at Thr222, Thr226, and Ser230 by GSK-3 may be required for adipogenesis (4). The two forms of C/EBPβ, 38 kDa liver activating protein (LAP) and the 20 kDa liver inhibitory protein (LIP), may result from alternative translation. The 38 kDa LAP protein is a transcriptional activator while LIP may inhibit C/EBPβ transcriptional activity (5). Phosphorylation of C/EBPβ at distinct sites stimulates its transcriptional activity (6-8). Phosphorylation at the rat-specific site Ser105 in C/EBPβ appears essential for C/EBPβ activation in rat (9). C/EBPδ protein is highly expressed in adipose tissue, lung, and intestine (10). Increased expression of C/EBPδ mRNA levels during adipogenesis suggests that C/EBPδ plays an important role in positively regulating adipogenesis (10,11). C/EBPδ is expressed in the mammalian nervous system and plays an important role in long-term memory (10,12). CHOP is a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominant-negative manner (13). CHOP expression is induced by cellular stresses, including starvation; induced CHOP suppresses cell cycle progression from G1 to S phase (14). During ER stress, the level of CHOP expression is elevated and CHOP functions to mediate programmed cell death (15).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibodies.
Recommended Antibody Dilutions:
Western blotting  1:1000