Aiolos Antibody

• 产品编号：CST-12720S      品牌：Cell Signaling Technology       原厂货号：12720S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Aiolos抗体能够检测内源性Aiolos总蛋白水平。此多克隆抗体是通过合成肽免疫动物制备，这种合成肽与人源Aiolos蛋白Pro46周围残基相一致。抗体通过蛋白A和多肽亲和层析纯化。Aiolos是lkaro家族转录因子，由锌指结构组成，这些锌指结构介导DNA的结合，以及同源二聚体和与其它lkaro家族成员的异源二聚体的形成(1)。多种Aiolos亚型是通过部分编码氨基末端锌指结构的转录本选择性剪接产生的(2)。Aiolos是通过淋巴组织表达的，在成熟的B细胞和T细胞中表达水平最高(1)。Ikaros家族通过将染色质重塑复合物招募至DNA处控制淋巴细胞的发育(3)。来自缺失Aiolos的小鼠的B细胞中激活的阈值降低，增殖水平提高，IgG和IgB水平提高，并出现B细胞淋巴瘤(4)。在T细胞中，Aiolos通过抑制IL-2的表达有助于Th17细胞的分化(5)。Aiolos基因的改变主要出现在靠近单倍体急性淋巴细胞白血病中，并且这个包含Aiolos的基因位点可以增加风湿性关节炎和全身性红斑狼疮的易感性(6-8)。

1. Morgan, B. et al. (1997) EMBO J 16, 2004-13.
2. Liippo, J. et al. (2001) Eur J Immunol 31, 3469-74.
3. Kim, J. et al. (1999) Immunity 10, 345-55.
4. Wang, J.H. et al. (1998) Immunity 9, 543-53.
5. Quintana, F.J. et al. (2012) Nat Immunol 13, 770-7.
6. Holmfeldt, L. et al. (2013) Nat Genet 45, 242-52.
7. Kurreeman, F.A. et al. (2012) Am J Hum Genet 90, 524-32.
8. Lessard, C.J. et al. (2012) Am J Hum Genet 90, 648-60.
9. Li, X. et al. (2014) Cancer Cell 25, 575-89.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Aiolos Antibody recognizes endogenous levels of total Aiolos protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro46 of human Aiolos protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Aiolos is an Ikaros family transcription factor composed of several zinc fingers that mediate DNA binding and homodimerization or heterodimerization with other Ikaros family members (1). Multiple Aiolos isoforms are generated through alternative splicing of the portion of the transcript encoding the amino-terminal zinc fingers (2). Aiolos is expressed by lymphoid tissues, with highest expression levels seen in mature B and T cells (1). The Ikaros family controls lymphocyte development by recruiting chromatin remodeling complexes to DNA (3). B cells from mice lacking Aiolos have a reduced threshold for activation, increased proliferation, elevated IgG and IgE, and develop B cell lymphomas (4). In T cells, Aiolos contributes to Th17 cell differentiation by suppressing IL-2 expression (5). Alterations in the Aiolos gene are observed in near haploid acute lymphoblastic leukemia, and the genetic locus containing Aiolos is linked to increased susceptibility to rheumatoid arthritis and systemic lupus erythematosus (6-8).

1. Morgan, B. et al. (1997) EMBO J 16, 2004-13.
2. Liippo, J. et al. (2001) Eur J Immunol 31, 3469-74.
3. Kim, J. et al. (1999) Immunity 10, 345-55.
4. Wang, J.H. et al. (1998) Immunity 9, 543-53.
5. Quintana, F.J. et al. (2012) Nat Immunol 13, 770-7.
6. Holmfeldt, L. et al. (2013) Nat Genet 45, 242-52.
7. Kurreeman, F.A. et al. (2012) Am J Hum Genet 90, 524-32.
8. Lessard, C.J. et al. (2012) Am J Hum Genet 90, 648-60.
9. Li, X. et al. (2014) Cancer Cell 25, 575-89.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.