Endodermal Lineage Marker Antibody Sampler Kit

• 产品编号：CST-12693S      品牌：Cell Signaling Technology       原厂货号：12693S
• 产品分类：抗体 > 一抗 > 复合抗体试剂盒
• 应用分类：

其它组分：

描述：

每个抗体都能检测对应的内源性靶蛋白。Sall4 (D16H12) Rabbit mAb兔单抗可以识别内源性总的Sall4A 和Sall4B蛋白。合成对应人AFP的羧基端残基序列，或对应人FoxA2/HNF3β的Gly138临近氨基酸残基序列，对应人GATA-6蛋白氨基端残基序列，对应人HNF4α蛋白序列，对应PDGFRα胞外结构域的重组蛋白，或对应人Sall4蛋白Ala311临近残基序列的肽段免疫动物获得单克隆抗体。使用对应人N-cadherin的氨基端序列或人EOMES蛋白的氨基端及其邻近氨基酸序列的多肽免疫动物获得多抗。多克隆抗体由结合蛋白A和肽段亲和层析技术纯化得到。Endodermal Lineage Marker Antibody Sampler试剂盒提供了一种经济的方式检测内胚层发育时期表达的蛋白。该试剂盒包含的每个一抗的抗体量足够进行四次western blot实验。哺乳动物胚胎发育时期会发育两个内胚系，囊胚期胚胎原始内胚层和原肠胚形成明确的内胚层。原始内胚层产生了涵盖脏层和壁层内胚层的胚外谱系涵盖脏层和壁层内胚层。内胚层通过形成气管，食道，肺，胃和肠道的上皮衬里而形成了呼吸道和胃肠道，并且是腺体，包括甲状腺，胸腺，胰腺和肝的主要成分(1)。了解调节早期内胚命运的分子机制是干细胞研究开创性的进步，因为它们哺乳动物发育过程中连接了从多潜能性定型到内胚层的过渡，有助于临床上相关的细胞类型的产生。FoxA2/HNF3β是一个对小鼠内胚层和中胚层结构发育至关重要的转录因子(2,3)。EOMES在原肠胚形成中促进内胚层的各结构形成(4)。内胚层中肝脏分化的标志包括α-fetoprotein (AFP)和N-cadherin的表达(5,6)。HNF4α涉及内脏内胚层的分化。GATA-6位于HNF4转录级联反应的上游，调控了内脏内胚层的分化，也是建立内胚层来源的支气管上皮细胞所必须的(7)。Sall4对于内细胞团形成原始内胚层是必须的。已有报道额外胚胎干细胞系不能由缺失Sall4的囊胚中形成(8)。PDGFRα表达在整个胚胎发育期的原始内胚层衍生物中(9)。

原厂资料：

Specificity / Sensitivity

Each antibody recognizes endogenous total levels of its specific target protein. Sall4 (D16H12) Rabbit mAb recognizes endogenous levels of total Sall4A and Sall4B proteins.

Source / Purification

Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human AFP protein, residues surrounding Gly138 of human FoxA2/HNF3β protein, residues near the amino terminus of human GATA-6 protein, the sequence of human HNF4α protein, a recombinant protein corresponding to the PDGF receptor α extracellular domain, or residues surrounding Ala311 of human Sall4 protein. Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human N-cadherin protein or near the amino terminus of human EOMES protein. Polyclonal antibodies are purified by protein A and peptide affinity chromatography.

Description

The Endodermal Lineage Marker Antibody Sampler Kit provides an economical means of evaluating proteins expressed during endoderm development. This kit contains enough antibody to perform four western blot experiments per primary antibody.

Background

Two endodermal lineages develop during mammalian embryogenesis, the primitive endoderm of the blastocyst stage embryo and the definitive endoderm at gastrulation. The primitive endoderm gives rise to extra-embryonic lineages encompassing the visceral and the parietal endoderm. The definitive endoderm contributes to the respiratory and gastrointestinal tracts by forming the epithelial lining of the trachea, esophagus, lungs, stomach and intestines, and is a major component of many glands, including thyroid, thymus, pancreas and liver (1). Understanding molecular mechanisms that regulate early endodermal fates is seminal for the advance of stem cell research as they connect the transition from pluripotency to endoderm specification during mammalian development and contribute to the generation of clinically relevant cell types. FoxA2/HNF3β is a transcription factor essential for development of the endoderm and midline structures in mouse embryos (2,3). EOMES acts during gastrulation to promote the specification of the definitive endoderm (4). Markers of hepatic differentiation in the endoderm include expression of α-fetoprotein (AFP) and N-cadherin (5,6). HNF4α is involved in the differentiation of the visceral endoderm. GATA-6 lies upstream of HNF4 in a transcriptional cascade that regulates differentiation of the visceral endoderm and is also required for the establishment of the endodermally derived bronchial epithelium (7). Sall4 is required for the formation of the primitive endoderm from inner cell mass. It has been reported that extra-embryonic stem cell lines cannot be formed in Sall4-deficient blastocysts (8). PDGF receptor α is expressed in primitive endoderm derivatives throughout embryogenesis (9).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.