PEX5 Pure 50/PEX5 50ug

Pex genes encode proteins called peroxins that are involved in protein transport from the cytoplasm to peroxisomes. Import of peroxisomal

matrix proteins depends on two peroxisomal targeting signals, PTS1 and PTS2. PTS1 is a C-terminal tripeptide of Ser-Lys-Leu and PTS2 is a

cleavable N-terminal signal. Two peroxin receptors, PEX5 and PEX7, specifically bind peroxisomal matrix proteins that contain the PTS1 and

PTS2 signal, respectively. Both receptors have repetitive motifs that belong to structural families. PEX5 contains seven tetratricopeptide

repeats (TPR), while PEX7 has six WD motifs. PEX5 is bound by the SH3 domain of the membrane-bound peroxin PEX13. This may

facilitate docking of PEX5-peroxisome protein complexes to the peroxisomal membrane. In addition, PEX12 and PEX14 bind PEX5 and may

act downstream of the docking process to promote protein import. After PEX5-mediated import of the peroxisomal matrix protein, PEX4

recycles PEX5 from the peroxisome to the cytoplasm. The ubiquitous expression of PEX5 indicates that PEX5-mediated import of

PTS1-containing proteins is commonly used by a variety of cell types.

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.

2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.