Ms CD117 BV605 2B8 50ug

• 产品编号：BD-563146      品牌：BD-Pharmingen       原厂货号：563146
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

The 2B8 monoclonal antibody specifically binds to CD117 (c-Kit), a transmembrane tyrosine-kinase receptor that is encoded by the Kit gene
(formerly dominant white spotting, W). The c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor) encoded by
the Kit1 gene (formerly steel, SI), is a co-mitogen for hematopoietic stem cells, myeloerythroid progenitors and a mast-cell differentiation
factor. The KitW and Kit1SI mutant alleles have similar pleiotropic effects on the development of melanocytes, germ cells, and the
hematopoietic system. In the adult bone marrow, CD117 is expressed on hematopoietic progenitor cells, including CD90 (Thy-1) low,
TER-119-, CD45R/B220-, CD11b (Mac-1)-, Ly-6G (Gr-1)-, CD4-, CD8-, and Sca-1 (Ly-6A/E)+ multipotent hemotopoietic stem cells,
progenitors committed to myeliod and/or erythroid lineages, and precursors of B and T lymphocytes. This widespread expression of CD117 in
hematopoietic precursors is consistent with the participation of c-Kit and its ligand in the regulation of several hematopoietic lineages.
Intrathymic expression of c-Kit and c-Kit ligand suggest that CD117 is also involved in the regulation of some events during the development
of T lymphocytes. CD117 is also expressed by mast cells and by dendritic cells found in the periarteriolar lymphocytoc sheaths (T-cell areas)
of splenic white pulp. The mAb 2B8 reportedly does not block the action of c-Kit. This clone 2B8 had been reported that there was
cross-reactivity with rat.
This antibody is conjugated to BD Horizon BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of
407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD
Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the
acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon
PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness
equivalent to PE conjugates and can be used as a third color off of the violet laser.
 
For optimal and reproducible results, BD Horizon Brilliant Stain Buffer should be used anytime two or more BD Horizon Brilliant dyes are
used in the same experiment. Fluorescent dye interactions may cause staining artifacts which may affect data interpretation. The BD Horizon
Brilliant Stain Buffer was designed to minimize these interactions. More information can be found in the Technical Data Sheet of the BD
Horizon Brilliant Stain Buffer (Cat. No. 563794)

原厂资料：

注意事项：

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing. .
3.Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.