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Canine C-Reactive Ptein (CRP) ELISA Kit

 
包装: Kit
运保温度: Store undiluted at 4°C.
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描述:

The Canine C-Reactive Protein (CRP) ELISA Kit is designed for the detection and quantitation of canine CRP in canine serum. CRP is an 
acute-phase protein produced by the liver during conditions of inflammation, bacterial infection, or tissue trauma. Quantitation of CRP can be 
useful for the determination of inflammatory conditions that would be otherwise difficult to detect and monitor. This ELISA for canine CRP 
is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes an antibody specific for canine CRP coated on a 96-well 
plate. Standards or samples are added to the wells, and any canine CRP present binds to the immobilized antibody. After the appropriate 
incubation, wells are washed and a horseradish peroxidase conjugated anti-canine CRP is added to produce an antibody-antigen-antibody 
"sandwich". Following another incubation period, the wells are washed again and a substrate solution is added, which produces a blue color 
in direct proportion to the amount of CRP present in the initial sample (development of a blue color indicates a positive reaction while 
negative reactions appear colorless). The reaction is interrupted with the Stop Solution, which changes the color from blue to yellow (negative 
reactions remain colorless or faintly yellow). The color is then measured at a wavelength of 450 nm (absorbance) on a spectrophotometer or 
on an ELISA reader. 


注意事项:

1.Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
3.Store undiluted at 4°C.


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