AHP851Z is specific for bovine interleukin-1 beta (IL-1 beta).
IL-1 beta is principally expressed by macrophages, and plays an important immunomodulatory role in the bovine immune response.
AHP851Z recognises both native and recombinant bovine IL-1 beta. No detectable cross-reactivity was observed with human, mouse or rat IL-1 beta.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1ug/ml - 10ug/ml
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Storage
Store at -20oC only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to bovine IL-1 beta were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography on protein G.
This antibody may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA system, in combination withAHP851Bas the detection antibody.PBP008may be used as a standard.