AHP805GA is specific for human calpain-10. Calpain-10 is a calcium activated protease, which is expressed ubiquitously. Calpain-10 differs from other calpains in that domain IV contains no EF hand motifs. Domain IV for calpain-10 is called the T domain. AHP805GA recognises this T domain, so will detect both the latent and active forms of calpain-10.
Calpain-10 is reported to be implicated in type 2 diabetes mellitus.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Paraffin
Western Blotting
1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own using appropriate negative/positive controls.
Western Blotting
Under reducing conditions AHP805GA detect major bands at 78kD and 66kD.
Storage
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human calpain-10 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
(A full Health and Safety assessment is available upon request) This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
