AHP2215 recognises human placental alkaline phosphatase, a membrane bound enzyme normally synthesized by syncytiotrophoblasts of the placenta. It is also often found in malignant tumours of germ cell origin such as seminomas.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
10ug/ml -
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Immunohistology
* This product does not require protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to human Placental Alkaline Phosphatase were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
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