AHP1870 recognises calretinin, a 29kDa calcium binding protein found mostly in the central nervous system and retina. It is a cytoplasmic protein involved in the regulation of calcium concentration, which is important in the regulation of a large variety of neuronal functions, including neurotransmitter release, ionic channel permeability, enzyme activity, and gene transcription. Mice deficient in calretinin show impairment in motor coordination.
Calretinin has also been shown as a sensitive and specific marker for mast cells.
Species Cross-Reactivity
Target Species
Cross Reactivity
Human
Expected from Sequence
Mouse
Expected from Sequence
Primate
Expected from Sequence
Rat
Yes
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
1/500
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1870 detects a band of approximately 29kDa in rat cerebellum.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Antiserum Preparation
Antisera to Calretinin were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG was prepared from whole serum by Protein A chromatography.
京公网安备11010802025653 版权所有：北京逸优科技有限公司
