AHP1233 recognises glycogen synthase 3 (GSK3) beta phosphorylated at serine 9. This is a 46 kDa member of the serine/threonine protein kinase family. Two isforms of GSK3 exist, alpha and beta. GSK3 beta is expressed in the testis, thymus, prostate, and ovary as well as being expressed at low levels in lung, brain and kidney tissue.
GSK3 beta is involved in many signalling pathways including energy metabolism, body pattern formation and microtubule growth and stability (which aids axon growth). GSK3 beta is also thought to be involved in oncogenesis and the hormonal control of several regulatory proteins including glycogen synthase.
Phosphorylation of serine-9 inhibits GSK3 beta function.
Species Cross-Reactivity
Target Species
Cross Reactivity
Bovine
Expected from Sequence
Chicken
Expected from Sequence
Dog
Expected from Sequence
Human
Expected from Sequence
Mouse
Expected from Sequence
Primate
Expected from Sequence
Xenopus
Expected from Sequence
Zebrafish
Expected from Sequence
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Flow Cytometry(1)
Immunohistology - Frozen
Immunohistology - Paraffin
Western Blotting(2)
1/1000
(1)
For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
(2)
Special conditions apply. See *
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1233 detects a band of approximately 46kDa in rat cortex cell lysates.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Antiserum Preparation
Antisera to rat GSK3 beta (pSer9) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
京公网安备11010802025653