2470-5386F detects cytomegalovirus early nuclear antigen. Cytomegalovirus is an enveloped double stranded DNA virus which in humans (HCMV) is also commonly known as human herpesvirus 5 (HHV-5). Early HCMV proteins are expressed in the nucleus of infected cells within 3 to 24 hours of infection, prior to viral DNA replication.
2470-5386F is also reactive with MRC-5 cells infected with CMV, but not uninfected cells.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Immunohistology
Suitable for methacam fixed, paraffin wax embedded sections as well as acetone fixed CMV infected cells or frozen sections.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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