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INV110 OS KT 20 RXN

 
包装:
运保温度: -80°C
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其它组分:

chemically competent INV110 E. coli【包装:21×50 μl】
Supercoiled pUC19 plasmid DNA【包装:20 μl】
Recovery Medium【包装:10 ml】


描述:

INV110 E. coli are derived from JM110 E. coli and are specifically designed for the growth and purification of plasmid DNA that will be digested with dam or dcm methylation-sensitive restriction enzymes. INV110 is a significant improvement over the JM110 strain. INV110 has been engineered to carry an endA1 mutation, which eliminates the non-specific endonuclease Endonuclease I, for improved plasmid DNA preparation. In addition, the INV110 genotype features:

  • Blue/white screening of recombinant clones (lacZΔM15)
  • Reduced cleavage of foreign methylated DNA for improved transformation of genomic DNA [Δ(mcrC-mrr)]
  • F´ episome for phage infection and rescue of single-strand DNA


注意事项:

One Shot® INV110 Chemically Competent E. coli contains 10 transformations of chemically competent cells in One Shot® format. Store at -80°C.


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