MKK3/MKK6 Control Cell Extracts

• 产品编号：CST-9233S      品牌：CST       原厂货号：9233S
• 产品分类：蛋白质表达与纯化 > 蛋白质样本制备 > 细胞组织粗提物与裂解物
• 应用分类：

描述：

非磷酸化的MKK3/MKK6细胞提取物: 未经处理的NIH/3T3细胞总提取物，作为阴性对照。细胞提取物加入到SDS样品缓冲液中。磷酸化的MKK3/MKK6细胞提取物:紫外照射的NIH/3T3细胞总提取物，作为阳性对照。细胞提取物加入到SDS样品缓冲液中。作为对照，CST建议使用20 µl磷酸化和非磷酸化的MKK3/MKK6对照提取物。MKK3和MKK6是两种密切相关的双特异性蛋白激酶，能够激活p38 MAPK（1-5）。MKK3和MKK6能够通过磷酸化p38 MAPK的活化位点Thr-Gly-Tyr从而使p38 MAPK激活，但不能磷酸化或激活Erk1/2或SAPK/JNK。p38 MAPK的磷酸化能够显著提高其磷酸化蛋白底物的能力，这些底物包括ATF-2和Elk-1。MKK3和MKK6能被不同形式的细胞压力和炎症细胞因子激活（4,5）。通过磷酸化MKK3的Ser189和Thr193位点(2)以及MKK6的Ser207和Thr211位点（4,5）可以激活MKK3和MKK6。

1. Derijard, B. et al. (1995) Science 267, 682-685.
2. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
3. Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384.
4. Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255.
5. Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.

原厂资料：

Description

Nonphosphorylated MKK3/MKK6 Control Cell Extracts: Total cell extracts from NIH/3T3 cells, prepared without treatment, serve as a negative control. Supplied in SDS Sample Buffer 
 
 Phosphorylated MKK3/MKK6 Control Cell Extracts: Total cell extracts from NIH/3T3 cells, prepared with UV light treatment, serve as a positive control. Supplied in SDS Sample Buffer.

Applications

As controls, CST recommends using 20 µl of phosphorylated and nonphosphorylated MKK3/MKK6 control cell extracts.

Background

MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site Thr-Gly-Tyr but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation of serine and threonine residues at sites Ser189 and Thr193 for MKK3 (2) and Ser207 and Thr211 for MKK6 (4,5).

1. Derijard, B. et al. (1995) Science 267, 682-685.
2. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
3. Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384.
4. Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255.
5. Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.

注意事项：

Storage: Supplied in SDS Sample Buffer: 62.5 mM Tris-HCl
(pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT,
0.01% w/v phenol red or bromophenol blue. Store at –20°C.