Phospho-MCM2 (Ser139) Antibody

• 产品编号：CST-8861S      品牌：CST       原厂货号：8861S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

 Phospho-MCM2 (Ser139) Antibody能够识别内源性丝氨酸（139位）磷酸化的MCM2蛋白。 该多克隆抗体是由合成的人源的针对MCM2蛋白丝氨酸（139位）的磷酸化肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。微小染色体维持（MCM）蛋白2-7是DNA复制起始和延伸所需的6个相关蛋白家族。 MCM2到MCM7蛋白相互结合，形成异源六聚体MCM复合物，被认为充当了DNA复制叉的复制解旋酶（1-5）。该复合体也是前复制复合体(pre-RC)的关键组成部分(回顾1)。Cdc6 和 Cdt1招募MCM复合体至有丝分裂末期/G1早期的起始识别复合体(ORC)形成pre-RC，促进DNA复制(2中已论述)。MCM2, MCM3, MCM4和MCM6亚单位的磷酸化似乎调节MCM复合体的活性和DNA合成的启动(6-8)。DNA复制时MCM蛋白被清除，通过pre-RC重构的抑制引起染色质未识别化。染色质批准允许每个细胞循环DNA复制一次，有助于保证基因选择和避免恶性肿瘤细胞的产生(回顾3)。研究亦表明MCM复合体通过保护复制叉的结构参与检验点控制，而且可以招募捕获后的检验点蛋白协助复制重启(回顾3,9)。

1. Lei, M. and Tye, B.K. (2001) J Cell Sci 114, 1447-54.
2. Lygerou, Z. and Nurse, P. (2000) Science 290, 2271-3.
3. Forsburg, S.L. (2004) Microbiol Mol Biol Rev 68, 109-31.
4. Tye, B.K. and Sawyer, S. (2000) J Biol Chem 275, 34833-6.
5. Labib, K. et al. (2000) Science 288, 1643-7.
6. Charych, D.H. et al. (2008) J Cell Biochem 104, 1075-86.
7. Masai, H. et al. (2006) J Biol Chem 281, 39249-61.
8. Lin, D.I. et al. (2008) Proc Natl Acad Sci USA 105, 8079-84.
9. Tsuji, T. et al. (2006) Mol Biol Cell 17, 4459-72.
10. Bailis, J.M. et al. (2008) Mol Cell Biol 28, 1724-38.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Dog

Specificity / Sensitivity

Phospho-MCM2 (Ser139) Antibody recognizes endogenous levels of MCM2 protein only when phosphorylated at Ser139. 

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser139 of human MCM2 protein. Antibodies are purified by protein A and peptide affinity chromatography. 

Background

The minichromosome maintenance (MCM) 2-7 proteins are a family of six related proteins required for the initiation and elongation of DNA replication. MCM2-7 bind together to form the heterohexameric MCM complex that is thought to act as a replicative helicase at the DNA replication fork (1-5). This complex is also a key component of the pre-replication complex (pre-RC) (reviewed in 1). Cdc6 and CDT1 recruit the MCM complex to the origin recognition complex (ORC) during late mitosis/early G1 phase forming the pre-RC and licensing the DNA for replication (reviewed in 2). Phosphorylation of the MCM2, MCM3, MCM4, and MCM6 subunits appears to regulate MCM complex activity and the initiation of DNA synthesis (6-8). MCM proteins are removed during DNA replication, causing chromatin to become unlicensed through inhibition of pre-RC reformation. Licensing of the chromatin permits the DNA to replicate only once per cell cycle, thereby helping to ensure that genetic alterations and malignant cell growth do not occur (reviewed in 3). Studies have shown that the MCM complex is involved in checkpoint control by protecting the structure of the replication fork and assisting in restarting replication by recruiting checkpoint proteins after arrest (reviewed in 3,9). 

1. Lei, M. and Tye, B.K. (2001) J Cell Sci 114, 1447-54.
2. Lygerou, Z. and Nurse, P. (2000) Science 290, 2271-3.
3. Forsburg, S.L. (2004) Microbiol Mol Biol Rev 68, 109-31.
4. Tye, B.K. and Sawyer, S. (2000) J Biol Chem 275, 34833-6.
5. Labib, K. et al. (2000) Science 288, 1643-7.
6. Charych, D.H. et al. (2008) J Cell Biochem 104, 1075-86.
7. Masai, H. et al. (2006) J Biol Chem 281, 39249-61.
8. Lin, D.I. et al. (2008) Proc Natl Acad Sci USA 105, 8079-84.
9. Tsuji, T. et al. (2006) Mol Biol Cell 17, 4459-72.
10. Bailis, J.M. et al. (2008) Mol Cell Biol 28, 1724-38.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.