Lck (V49) Antibody

• 产品编号：CST-2714S      品牌：CST       原厂货号：2714S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Lck (V49) Antibody 能够检测内源Lck蛋白总体水平。此抗体不与Src家族的其它成员交叉反应。此多克隆抗体是通过合成人源对应的Lck Val49位点周围的肽段来免疫动物而获得。抗体是通过多肽亲和层析纯化。Src酪氨酸激酶蛋白家族包括Src, Lyn, Fyn, Yes, Lck, Blk 和 Hck, 其对真核细胞的生长和分化过程中起了重要的作用(1)。Src的活性是通过两个位点酪氨酸的磷酸化进行调节的, 但是具有相反地作用。在激酶活性环内的Tyr416 位点的磷酸化增加酶的活性,通过Csk磷酸化C-末端的Tyr527位点降低酶的活性(2)。Lck对T淋巴细胞的激活和分化起了重要的作用(3,4)。 在C-末端上Tyr505位点的磷酸化降低其催化活性, 同时，磷酸化Tyr394位点增加Lck的活性(5)。

1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
2. Hunter, T. (1987) Cell 49, 1-4.
3. Molina, T.J. et al. (1992) Nature 357, 161-164.
4. Straus, D.B. and Weiss, A. (1992) Cell 70, 585-593.
5. Chow, L.M. et al. (1993) Nature 365, 156-160.

原厂资料：

Specificity / Sensitivity

Lck (V49) Antibody detects endogenous levels of total Lck protein. This antibody does not cross-react with other src family proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val49 of human Lck. Antibodies are purified by peptide affinity chromatography.

Background

The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2).Lck is essential for T-lymphocyte activation and differentiation (3,4). Phosphorylation of Tyr505 in the carboxy-terminal tail of Lck downregulates its catalytic activity, while phosphorylation of Tyr394 leads to an increase in Lck activity (5).

1. Thomas, S.M. and Brugge, J.S. (1997) Annu Rev Cell Dev Biol 13, 513-609.
2. Hunter, T. (1987) Cell 49, 1-4.
3. Molina, T.J. et al. (1992) Nature 357, 161-164.
4. Straus, D.B. and Weiss, A. (1992) Cell 70, 585-593.
5. Chow, L.M. et al. (1993) Nature 365, 156-160.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody