CRABP1 (D5W9A) Rabbit mAb

• 产品编号：CST-13206S      品牌：CST       原厂货号：13206S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CRABP1 (D5W9A) Rabbit mAb兔单抗可以识别内源性CRABP1总蛋白水平。该抗体与其他细胞内脂质结合蛋白家族成员CRBP1和CRBP2没有交叉反应。单克隆抗体由合成肽段免疫动物产生，该肽段与人CRABP1蛋白氨基端氨基酸序列一致。维生素A被称为视黄酸，能生成多个物种的生物活性亲脂代谢产物，这在许多生理过程，如视觉和胚胎发育起着至关重要的作用。细胞内，所有的反式视黄酸都和任一种细胞视黄酸结合蛋白1(CRABP1)或细胞视黄酸结合蛋白2(CRABP2)具有高亲和性，这有助于它在亲水性胞浆小室中溶解。人CRABPs属于细胞内脂质结合蛋白家族(iLBP)，该蛋白的蛋白水平相似性为74%，并且在不同物种体内CRABP高度保守。研究指出敲除Crabp1不会致死但会发生手臂发育缺陷(1)，提示CRABP1起着在发育中的枝芽部位建立视黄酸浓度梯度的作用。尽管CRABP1是如何在体内调控视黄酸浓度梯度的尚不清楚，研究提示CRABP1可以增强细胞内视黄酸代谢酶的活性，从而钝化细胞对视黄酸的反应(2-4)。

1. Lampron, C. et al. (1995) Development 121, 539-48.
2. Fujii, H. et al. (1997) EMBO J 16, 4163-73.
3. Boylan, J.F. and Gudas, L.J. (1992) J Biol Chem 267, 21486-91.
4. Boylan, J.F. and Gudas, L.J. (1991) J Cell Biol 112, 965-79.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat, Chicken, Bovine

Specificity / Sensitivity

CRABP1 (D5W9A) Rabbit mAb recognizes endogenous levels of total CRABP1 protein. This antibody does not cross-react with other intracellular lipid-binding protein family members, CRBP1 and CRBP2.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human CRABP1 protein.

Background

Vitamin A gives rise to multiple species of biologically active lipophilic metabolites, known as retinoids, which play a critical role in numerous physiological processes such as vision and embryonic development. Intracellularly, all-transretinoic acid is bound with high affinity to either cellular retinoic acid-binding protein 1 (CRABP1) or cellular retinoic acid-binding protein 2 (CRABP2), which aids in its solubilization within the aqueous cytosolic compartment. Belonging to the intracellular lipid-binding protein family (iLBP), the human CRABPs are 74% identical at the protein level and each CRABP is highly conserved across multiple species. Research studies have shown that knockout of Crabp1 is not lethal but results in defects in limb development (1), suggesting that CRABP1 plays a role in establishing retinoic acid concentration gradients in the developing limb bud. Although it remains unclear how CRABP1 may regulate the formation of retinoic acid gradients in vivo, research studies have suggested that CRABP1 can enhance the activities of intracellular retinoic acid-metabolizing enzymes, thus blunting cellular responses to retinoic acid (2-4).

1. Lampron, C. et al. (1995) Development 121, 539-48.
2. Fujii, H. et al. (1997) EMBO J 16, 4163-73.
3. Boylan, J.F. and Gudas, L.J. (1992) J Biol Chem 267, 21486-91.
4. Boylan, J.F. and Gudas, L.J. (1991) J Cell Biol 112, 965-79.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody