EVL Antibody

• 产品编号：CST-12536S      品牌：CST       原厂货号：12536S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

EVL抗体可以识别内源性总EVL蛋白。多抗由合成肽段免疫动物产生，该肽段与人EVL蛋白362位丝氨酸邻近残基序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。Ena/VASP-like (EVL)蛋白是Ena/VASP家族的一员，涉及了肌动蛋白丝相关的细胞骨架重构和细胞极化功能包括轴突导向和迁移细胞的板状伪足的形成（1,2,3）。EVL蛋白序列包括一个N末端EVH1结构域，一个富含脯氨酸的SH3结合结构域，一个C末端EVH2结构域。EVL结构域与G和F-actin结合介导了肌动蛋白丝的集结和聚集（4）。研究显示EVL也通过与RAD51结合调控DAN的修复（5）。EVL通过EVH2结构域在DSB修复途径中发挥作用，这个结构域拥有DNA结合和RAD51结合活性，因此协助同源DNA重组（6,7）。研究显示EVL表达上调与人类乳腺癌的临床分期有关,可能涉及了人类乳腺癌的入侵和/或转移（8）。

1. Michael, M. et al. (2010) Curr Biol 20, 783-91.
2. Goh, K.L. et al. (2002) Curr Biol 12, 565-9.
3. Lebrand, C. et al. (2004) Neuron 42, 37-49.
4. Laurent, V. et al. (1999) J Cell Biol 144, 1245-58.
5. Takaku, M. et al. (2009) J Biol Chem 284, 14326-36.
6. Takaku, M. et al. (2011) J Biochem 149, 721-9.
7. Takaku, M. et al. (2009) FEBS J 276, 5841-8.
8. Hu, L.D. et al. (2008) Oncol Rep 19, 1015-20.

原厂资料：

Specificity / Sensitivity

EVL Antibody recognizes endogenous levels of total EVL protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser362 of human EVL protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Ena/VASP-like (EVL) protein is a member of the Ena/VASP family and is involved in actin-associated cytoskeleton remodeling and cell polarity activities including axon guidance and lamellipodia formation in migrating cells (1,2,3). The EVL protein sequence contains an N-terminal EVH1 domain, a Pro-rich SH3 binding domain, and a C-terminal EVH2 domain. EVL domain interactions with G- and F-actin mediates actin nucleation and polymerization (4). Research studies have shown that EVL also regulates DNA repair by direct interaction with RAD51 (5). EVL may function in the DSB repair pathway through the EVH2 domain, which possesses DNA-binding and RAD51 binding activity, thereby coordinating homologous DNA recombination (6,7). Research studies have shown EVL expression is up-regulated in human breast cancer associated with clinical stages and may be implicated in invasion and/or metastasis of human breast cancer (8).

1. Michael, M. et al. (2010) Curr Biol 20, 783-91.
2. Goh, K.L. et al. (2002) Curr Biol 12, 565-9.
3. Lebrand, C. et al. (2004) Neuron 42, 37-49.
4. Laurent, V. et al. (1999) J Cell Biol 144, 1245-58.
5. Takaku, M. et al. (2009) J Biol Chem 284, 14326-36.
6. Takaku, M. et al. (2011) J Biochem 149, 721-9.
7. Takaku, M. et al. (2009) FEBS J 276, 5841-8.
8. Hu, L.D. et al. (2008) Oncol Rep 19, 1015-20.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody